Year: 2017 | Month: April | Volume 10 | Issue 2
Effect of Silver Nitrate on Leaf Abscission in Culture during Establishment of Explants of Custard Apple (Annona squamosa l.) cv. Balanagar
Abstract:
The present investigation was carried out on “Micropropagation in custard apple (Annona squamosa L.)” with objective to develop commercially feasible and economically viable protocol for micropropagation technique of custard apple. While standardization of micropropagation in custard apple, Effect of silver nitrate on leaf abscission in culture during establishment of explants of custard apple cv. Balanagar were examined. In order to control leaf abscission silver nitrate at concentrations of 0, 2.5, 5 and 10 mg/l was tested to medium MS + 0.5 mg/l BAP + 0.5 mg/l KIN + 100mg/l CH. The results revealed that Silver nitrate significantly reduced leaf abscission in both shoot tip and axillary bud explants. The least leaf abscission was reported in treatment N3 (5 mg/l) followed by treatment N4 (10 mg/l) and N1 (2.5mg/l) in both shoot tip and axillary bud explants. Whereas, in treatment N1 (without silver nitrate) more than 80% leaf abscission was observed. The number of leaves produced by the explants was maximum in treatment N3 (5 mg/l) in both shoot tip (7.5) and axillary bud (8.5) explants. Whereas, when silver nitrate was applied at 10 mg/l (Treatment N4) this was phytotoxic and inhibited leaf production.
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